洛铂和顺铂对血管内皮细胞及肝、肾细胞损伤的体外实验研究

doi:10.3969/j.issn.1674-5671.2011.02.02

中国癌症防治杂志 ›› 2011, Vol. 3 ›› Issue (2): 103-.doi: 10.3969/j.issn.1674-5671.2011.02.02

洛铂和顺铂对血管内皮细胞及肝、肾细胞损伤的体外实验研究

黄文锋, 谢伟敏, 王洪学, 陆永奎, 周文献

出版日期:2011-08-03 发布日期:2011-08-03

HUANG Wen-Feng, XIE Wei-Min, WANG Hong-Xue, LU Yong-Kui, ZHOU Wen-Xian

Online:2011-08-03 Published:2011-08-03
Supported by:
国家自然科学基金资助项目（30960436）；广西卫生厅科研课题（Z2009240、Z2009251）

摘要/Abstract

摘要： 【摘要】目的分析洛铂（LBP）和顺铂（DDP）对传代培养的人脐静脉内皮细胞株（HUVECs）、人肝细胞株（QSG-7701）和人近端肾小管上皮细胞（HK-2细胞）的抑制作用，并初步探讨其可能机制。方法采用MTT法检测LBP和DDP对HUVECs、QSG-7701和HK-2细胞抑制作用的差异，并测定HK-2细胞培养液中丙二醛（MDA）和超氧化物歧化酶（SOD）含量，观察LBP和DDP对HK-2细胞脂质过氧化过程的影响。结果在相同浓度、相同作用时间下，DDP组对HK-2细胞抑制率明显高于LBP组（P＜0.05），而LBP组对HUVECs细胞的抑制率明显高于DDP组（P＜0.05）；LBP组和DDP组对QSG7701抑制率的差异无统计学意义（P＞0.05）；此外，与空白对照组比较，LBP组和DDP组均使HK-2细胞培养液中MDA含量明显升高，SOD活性明显降低（P均＜0.05），但LBP组和DDP组间差异无统计学意义（P＞0.05）。结论LBP和DDP均对肝、肾细胞有较强的抑制作用，LBP的肾细胞毒性较DDP低，LBP组对正常人脐静脉内皮细胞抑制作用明显强于DDP组，提示LBP可能具有更强的抑制血管形成的能力；氧化性损伤可能是两者造成肾毒性的机制。

关键词: 【关键词】铂类, HUVECs, QSG-7701, HK-2 , cells

Abstract: 【Abstract】ObjectiveTo study the damage effect of lobaplatin (LBP) and cisplatin (DDP) on human umbilical vein endothelia cell line (HUVECs)，human liver cell line (QSG7701) and human renal tubular epithelial cells (HK-2 cells)，and to explore the possible mechanismMethodsThe proliferation responses of HUVECs，QSG-7701 and HK2 cells treated with LBP or DDP in the concentration of 3.125ug/ml were observed by MTT assayIn addition，the concentrations of malondialdehyde (MDA) and superoxide dismutase (SOD) were determined in HK2 cellsResultsWith the same concentration and action time，the inhibition effect of DDP on HK2 cells was significantly higher than that of LBP (P＜0.05)，while the inhibition effect of LBP on HUVECs was significantly higher that that of DDP (P＜0.05)The inhibition rate of these two drugs on QSG7701 was not significantly different (P＞0.05)The level of MDA in LBPtreated group or DDPtreated group was significantly higher than that in control group (P＜0.05)，the level of SOD in both platinum groups was significantly lower than that in control group (P＜0.05)，while both the levels of MAD and SOD were not significantly different between LBPtreated group and DDPtreated group (both P＞0.05)Conclusion LBP and DDP displayed inhibitive effect on human hepatocytes and renal tubular epithelial cellsLBP might have stronger antiangiogenesis effect on HUVECs comparing to DDPOxidative damage may be a common mechanism of renal toxicity caused by LBP and DDP

Key words: 【Key words】 Platinum, HUVECs, QSG-7701, HK-2 , cells